Cloning and sequence analysis of Tibet mini-pig’ s MSTN gene / 中国比较医学杂志

Objective To amplify the encoding full-length sequence of Tibet mini-pigs myostatin ( MSTN) gene and analysis the sequence by bioinformatics software.Method The RNA of liver tissues from Tibet mini-pig was extracted, and reversely transcribed into cDNA.The gene coding region sequence of myostatin gene was amplified through RT-PCR, and then the purified product of PCR was ligated with a pMD19-T and transferred into the bacterium DH5αfor replication.The positive clones were screened and sequenced. The sequence characters were analyzed by using bioinformatics method and phylogeny evolution tree was constructed with other twelve species.Results The coding redion of MSTN gene was 1128bp, and coded 375 amino acids.The amino acid homology analysis showed that the homology rate of amino acid sequence was 99%.Conclusions Molecular phylogeny evolution indicated that it had a close relation with human, dog, banna minipig, sheep, goat, cattle, horse, chimpanzee, rat, mouse except chick and zebrafish, and the most closely related with banna minipig.

Cloning and Squence Analysis of Gene Coding for Dextransucrase from Leuconostoc mesenteroides / 微生物学通报

The desired DNA product of dsr1 and dsr2 was amplified from the total DNA of the L0309 screened in this lab by PCR with two pairs of gene specific primers.The segment of dsr1 and dsr2 was inserted into pUC19 vector and integrated into the entire gene of dsrx.The result of restriction endonuclease mapping and sequencing shows that 4566 bp of dsrx covers the entire open reading frame,encoding 1522 amino acid residues.There is an identity of 99% between the gene of U81374 and dsrx about their nuclear acid sequences.The homology of the deduced amino acid level amount to 98.49% with that of U81374.